Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with 2019 Novel Coronavirus Disease, United States.
Identifieur interne : 000347 ( Main/Exploration ); précédent : 000346; suivant : 000348Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with 2019 Novel Coronavirus Disease, United States.
Auteurs : Jennifer Harcourt ; Azaibi Tamin ; Xiaoyan Lu ; Shifaq Kamili ; Senthil K. Sakthivel ; Janna Murray ; Krista Queen ; Ying Tao ; Clinton R. Paden ; Jing Zhang ; Yan Li ; Anna Uehara ; Haibin Wang ; Cynthia Goldsmith ; Hannah A. Bullock ; Lijuan Wang ; Brett Whitaker ; Brian Lynch ; Rashi Gautam ; Craig Schindewolf ; Kumari G. Lokugamage ; Dionna Scharton ; Jessica A. Plante ; Divya Mirchandani ; Steven G. Widen ; Krishna Narayanan ; Shinji Makino ; Thomas G. Ksiazek ; Kenneth S. Plante ; Scott C. Weaver ; Stephen Lindstrom ; Suxiang Tong ; Vineet D. Menachery ; Natalie J. ThornburgSource :
- Emerging infectious diseases [ 1080-6059 ] ; 2020.
Abstract
The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.
DOI: 10.3201/eid2606.200516
PubMed: 32160149
Affiliations:
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Widen</name></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author><author><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G" last="Ksiazek">Thomas G. Ksiazek</name></author><author><name sortKey="Plante, Kenneth S" sort="Plante, Kenneth S" uniqKey="Plante K" first="Kenneth S" last="Plante">Kenneth S. Plante</name></author><author><name sortKey="Weaver, Scott C" sort="Weaver, Scott C" uniqKey="Weaver S" first="Scott C" last="Weaver">Scott C. Weaver</name></author><author><name sortKey="Lindstrom, Stephen" sort="Lindstrom, Stephen" uniqKey="Lindstrom S" first="Stephen" last="Lindstrom">Stephen Lindstrom</name></author><author><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name></author><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name></author><author><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. 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Sakthivel</name></author><author><name sortKey="Murray, Janna" sort="Murray, Janna" uniqKey="Murray J" first="Janna" last="Murray">Janna Murray</name></author><author><name sortKey="Queen, Krista" sort="Queen, Krista" uniqKey="Queen K" first="Krista" last="Queen">Krista Queen</name></author><author><name sortKey="Tao, Ying" sort="Tao, Ying" uniqKey="Tao Y" first="Ying" last="Tao">Ying Tao</name></author><author><name sortKey="Paden, Clinton R" sort="Paden, Clinton R" uniqKey="Paden C" first="Clinton R" last="Paden">Clinton R. Paden</name></author><author><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Uehara, Anna" sort="Uehara, Anna" uniqKey="Uehara A" first="Anna" last="Uehara">Anna Uehara</name></author><author><name sortKey="Wang, Haibin" sort="Wang, Haibin" uniqKey="Wang H" first="Haibin" last="Wang">Haibin Wang</name></author><author><name sortKey="Goldsmith, Cynthia" sort="Goldsmith, Cynthia" uniqKey="Goldsmith C" first="Cynthia" last="Goldsmith">Cynthia Goldsmith</name></author><author><name sortKey="Bullock, Hannah A" sort="Bullock, Hannah A" uniqKey="Bullock H" first="Hannah A" last="Bullock">Hannah A. Bullock</name></author><author><name sortKey="Wang, Lijuan" sort="Wang, Lijuan" uniqKey="Wang L" first="Lijuan" last="Wang">Lijuan Wang</name></author><author><name sortKey="Whitaker, Brett" sort="Whitaker, Brett" uniqKey="Whitaker B" first="Brett" last="Whitaker">Brett Whitaker</name></author><author><name sortKey="Lynch, Brian" sort="Lynch, Brian" uniqKey="Lynch B" first="Brian" last="Lynch">Brian Lynch</name></author><author><name sortKey="Gautam, Rashi" sort="Gautam, Rashi" uniqKey="Gautam R" first="Rashi" last="Gautam">Rashi Gautam</name></author><author><name sortKey="Schindewolf, Craig" sort="Schindewolf, Craig" uniqKey="Schindewolf C" first="Craig" last="Schindewolf">Craig Schindewolf</name></author><author><name sortKey="Lokugamage, Kumari G" sort="Lokugamage, Kumari G" uniqKey="Lokugamage K" first="Kumari G" last="Lokugamage">Kumari G. Lokugamage</name></author><author><name sortKey="Scharton, Dionna" sort="Scharton, Dionna" uniqKey="Scharton D" first="Dionna" last="Scharton">Dionna Scharton</name></author><author><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name></author><author><name sortKey="Mirchandani, Divya" sort="Mirchandani, Divya" uniqKey="Mirchandani D" first="Divya" last="Mirchandani">Divya Mirchandani</name></author><author><name sortKey="Widen, Steven G" sort="Widen, Steven G" uniqKey="Widen S" first="Steven G" last="Widen">Steven G. Widen</name></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author><author><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G" last="Ksiazek">Thomas G. Ksiazek</name></author><author><name sortKey="Plante, Kenneth S" sort="Plante, Kenneth S" uniqKey="Plante K" first="Kenneth S" last="Plante">Kenneth S. Plante</name></author><author><name sortKey="Weaver, Scott C" sort="Weaver, Scott C" uniqKey="Weaver S" first="Scott C" last="Weaver">Scott C. Weaver</name></author><author><name sortKey="Lindstrom, Stephen" sort="Lindstrom, Stephen" uniqKey="Lindstrom S" first="Stephen" last="Lindstrom">Stephen Lindstrom</name></author><author><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name></author><author><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name></author><author><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name></author></analytic><series><title level="j">Emerging infectious diseases</title><idno type="eISSN">1080-6059</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bullock, Hannah A" sort="Bullock, Hannah A" uniqKey="Bullock H" first="Hannah A" last="Bullock">Hannah A. Bullock</name><name sortKey="Gautam, Rashi" sort="Gautam, Rashi" uniqKey="Gautam R" first="Rashi" last="Gautam">Rashi Gautam</name><name sortKey="Goldsmith, Cynthia" sort="Goldsmith, Cynthia" uniqKey="Goldsmith C" first="Cynthia" last="Goldsmith">Cynthia Goldsmith</name><name sortKey="Harcourt, Jennifer" sort="Harcourt, Jennifer" uniqKey="Harcourt J" first="Jennifer" last="Harcourt">Jennifer Harcourt</name><name sortKey="Kamili, Shifaq" sort="Kamili, Shifaq" uniqKey="Kamili S" first="Shifaq" last="Kamili">Shifaq Kamili</name><name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G" last="Ksiazek">Thomas G. Ksiazek</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Lindstrom, Stephen" sort="Lindstrom, Stephen" uniqKey="Lindstrom S" first="Stephen" last="Lindstrom">Stephen Lindstrom</name><name sortKey="Lokugamage, Kumari G" sort="Lokugamage, Kumari G" uniqKey="Lokugamage K" first="Kumari G" last="Lokugamage">Kumari G. Lokugamage</name><name sortKey="Lu, Xiaoyan" sort="Lu, Xiaoyan" uniqKey="Lu X" first="Xiaoyan" last="Lu">Xiaoyan Lu</name><name sortKey="Lynch, Brian" sort="Lynch, Brian" uniqKey="Lynch B" first="Brian" last="Lynch">Brian Lynch</name><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name><name sortKey="Menachery, Vineet D" sort="Menachery, Vineet D" uniqKey="Menachery V" first="Vineet D" last="Menachery">Vineet D. Menachery</name><name sortKey="Mirchandani, Divya" sort="Mirchandani, Divya" uniqKey="Mirchandani D" first="Divya" last="Mirchandani">Divya Mirchandani</name><name sortKey="Murray, Janna" sort="Murray, Janna" uniqKey="Murray J" first="Janna" last="Murray">Janna Murray</name><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name><name sortKey="Paden, Clinton R" sort="Paden, Clinton R" uniqKey="Paden C" first="Clinton R" last="Paden">Clinton R. Paden</name><name sortKey="Plante, Jessica A" sort="Plante, Jessica A" uniqKey="Plante J" first="Jessica A" last="Plante">Jessica A. Plante</name><name sortKey="Plante, Kenneth S" sort="Plante, Kenneth S" uniqKey="Plante K" first="Kenneth S" last="Plante">Kenneth S. Plante</name><name sortKey="Queen, Krista" sort="Queen, Krista" uniqKey="Queen K" first="Krista" last="Queen">Krista Queen</name><name sortKey="Sakthivel, Senthil K" sort="Sakthivel, Senthil K" uniqKey="Sakthivel S" first="Senthil K" last="Sakthivel">Senthil K. Sakthivel</name><name sortKey="Scharton, Dionna" sort="Scharton, Dionna" uniqKey="Scharton D" first="Dionna" last="Scharton">Dionna Scharton</name><name sortKey="Schindewolf, Craig" sort="Schindewolf, Craig" uniqKey="Schindewolf C" first="Craig" last="Schindewolf">Craig Schindewolf</name><name sortKey="Tamin, Azaibi" sort="Tamin, Azaibi" uniqKey="Tamin A" first="Azaibi" last="Tamin">Azaibi Tamin</name><name sortKey="Tao, Ying" sort="Tao, Ying" uniqKey="Tao Y" first="Ying" last="Tao">Ying Tao</name><name sortKey="Thornburg, Natalie J" sort="Thornburg, Natalie J" uniqKey="Thornburg N" first="Natalie J" last="Thornburg">Natalie J. Thornburg</name><name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name><name sortKey="Uehara, Anna" sort="Uehara, Anna" uniqKey="Uehara A" first="Anna" last="Uehara">Anna Uehara</name><name sortKey="Wang, Haibin" sort="Wang, Haibin" uniqKey="Wang H" first="Haibin" last="Wang">Haibin Wang</name><name sortKey="Wang, Lijuan" sort="Wang, Lijuan" uniqKey="Wang L" first="Lijuan" last="Wang">Lijuan Wang</name><name sortKey="Weaver, Scott C" sort="Weaver, Scott C" uniqKey="Weaver S" first="Scott C" last="Weaver">Scott C. Weaver</name><name sortKey="Whitaker, Brett" sort="Whitaker, Brett" uniqKey="Whitaker B" first="Brett" last="Whitaker">Brett Whitaker</name><name sortKey="Widen, Steven G" sort="Widen, Steven G" uniqKey="Widen S" first="Steven G" last="Widen">Steven G. Widen</name><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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